Fig. 1

Morphological and molecular analysis of the internal genitalia of Masc-R females homozygous for BmdsxM mutation. Homozygous mutation in BmdsxM fully restored ovary development in Masc-R females. a, b The expression levels of BmdsxM (a) and BmdsxF (b) in animals with the indicated genotype were quantified by qRT-PCR. c, d Expression of Bmovo-1 (c) and Bm-nosO (d), which is important for oogenesis, was quantified by qRT-PCR to evaluate the effect of BmdsxM knockout on gonadal sexual development in Masc-R/+ females. Error bars indicate standard deviation. * indicates a significant difference, as determined by Welch’s t-test. e–g Morphological analysis of the internal genitalia in Masc-R female heterozygous for Bmdsx^MΔ7 (E), Masc-R female homozygous for Bmdsx^MΔ7 (F), and normal female animals (g). e, egg; mg, mucous gland; ov, oviduct; sp., spermatheca; sr, seminal receptacle; tl, testis-like tissue. h–j High magnification images showing the apical end of the ovariole in Masc-R female heterozygous for Bmdsx^MΔ7 h, Masc-R female homozygous for Bmdsx^MΔ7(I), and normal female animals j. The dotted line indicates the testis-like tissues