Table 3 Primer sequences for target genes
From: Notch signaling in mouse blastocyst development and hatching
Target gene | Sequence (5′ – 3′) | Product length (bp) | Accession no. |
|---|---|---|---|
Notch1 | Fwd: ACAGTAACCCCTGCATCCAC Rev.: GGTTGGACTCACACTCGTTG | 120 | NM_008714.3 |
Notch2 | Fwd: GACTGCACAGAAGACGTGGA Rev.: GCGTAGCCCTTCAGACACTC | 116 | NM_010928.2 |
Notch3 | Fwd: GTGTCAATGGTGGTGTCTGC Rev.: GCACACTCATCCACATCCAG | 103 | NM_008716.2 |
Notch4 | Fwd: GAGGGACACTCCACCTTTCA Rev.: CTGGTGCCTGACACAGTCAT | 93 | NM_010929.2 |
Delta-like1 | Fwd: GTTGTCTCCATGGCACCTG Rev.: TGCACGGCTTATGGTGAGTA | 111 | NM_007865.3 |
Delta-like4 | Fwd: GGAACCTTCTCACTCAACATCC Rev.: CTCGTCTGTTCGCCAAATCT | 141 | NM_019454.3 |
Jagged1 | Fwd: CCAGCCAGTGAAGACCAAGT Rev.: CAATTCGCTGCAAATGTGTT | 127 | NM_013822.5 |
Jagged2 | Fwd: AGTGCCATCTGGCTTTGAAT Rev.: CGCTGCACATGGGTTAGAG | 97 | NM_010588.2 |
Hes1 | Fwd: GCGAAGGGCAAGAATAAATG Rev.: TGTCTGCCTTCTCTAGCTTGG | 104 | NM_008235.2 |
Hes2 | Fwd: CGGATCAACGAGAGCCTAAG Rev.: GTCTGCCTTCTCCAACTTCG | 93 | NM_001301805.1; NM_008236.4 |
Sox2 | Fwd: GGTTCTTGCTGGGTTTTGATTCT Rev.: CCTTCCTTGTTTGTAACGGTCCT | 59 | NM_011443.4 |
Klf4 | Fwd: GCAGTCACAAGTCCCCTCTC Rev.: GACCTTCTTCCCCTCTTTGG | 93 | NM_010637.3 |
Oct4 | Fwd: TGGAGGAAGCCGACAACAAT Rev.: GCTGATTGGCGATGTGAGTG | 177 | NM_001252452.1; NM_013633.3 |
Cdx2 | Fwd: CTGGCTCCGCAGAACTTTGT Rev.: GGTGCGTAGCCATTCCAGTC | 170 | NM_007673.3 |
Cdca7 | Fwd: ACA TGC TGG TGA GAC AGA GGA A Rev.: TAT ATG CGG AAG GGT CAT GGA | 98 | NM_025866.3 |
Lgr5 | Fwd: CCC ATC CAA TTT GTT GGA GTA Rev.: GTG GCA GTT CCT GTC AAG TG | 113 | NM_010195.2 |
Rps29 | Fwd: CACGGTCTGATCCGCAAATAC Rev.: ACTAGCATGATCGGTTCCACTTG | 144 | NM_009093.2 |
Hprt1 | Fwd: GTCGTGATTAGCGATGATGAACC Rev.: GCAAGTCTTTCAGTCCTGTCCATAA | 128 | NM_013556.2 |