Fig. 5

Acute injection of eB2-Fc in the lateral ventricle leads to an increased neuronal production. a. Transverse sections of the neocortex of E15.5 or E18.5 control injected embryos (IgG) (n = 4) and embryos injected with eB2-Fc (n = 4) were immunostained for Tbr1 (red) and Satb2 (green). Scale bars: 50 μm. b. Quantification of the number of neurons in the neocortex of E15.5 and E18.5 embryos. The graph represents numbers of Tbr1+ and Satb2+ neurons in the lateral region of the neocortex in control and eB2-Fc injected embryos. c. Quantification of the number of Tbr1+ and Satb2+ neurons in the neocortex of E15.5 injected embryos (dorsal region of the neocortex). Data for each counted hemisphere are plotted. d. Transverse sections of the ventricular zone and subventricular zone of E15.5 control injected embryos (IgG) and embryos injected with eB2-Fc (dorsal region of the neocortex) were immunostained for Pax6 (red) and Tbr2 (green). Scale bars: 20 μm. e. Quantification of the number of Pax6+ and Tbr2+ progenitors in IgG (n = 4) and eB2-Fc (n = 5) injected embryos. Data for each counted hemisphere are plotted. Data are reported as mean ± SEM. Mann-Whitney statistical test (*P < 0.05; **P < 0.01)