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Table 1 Effects of ocu-miR-205 on proliferation, cell cycle and apoptosis of dermal papilla cells (DPCs; %)

From: The functions of ocu-miR-205 in regulating hair follicle development in Rex rabbits

Items

Group

R-MSE

P-value

Control

HBAD-GFP

HBAD-ocu-miR-205-GFP

HBAD-ocu-miR-205-5p-sponge-GFP

Proliferation of dermal papilla cells

 Optical density (OD) value

0.56 ± 0.01b

0.55 ± 0.01b

0.61 ± 0.01a

0.34 ± 0.01c

0.0356

< 0.0001

Cell cycle of dermal papilla cells

 Resting state/first gap (G0/G1)

87.79 ± 1.00a

84.68 ± 1.01b

77.71 ± 0.88c

85.57 ± 1.03ab

2.7795

< 0.0001

 Synthesis (S)

7.15 ± 0.85b

9.48 ± 0.95b

14.73 ± 0.62a

8.97 ± 0.68b

2.2232

< 0.0001

 Second gap/mitosis (G2/M)

5.07 ± 0.24b

5.37 ± 0.49b

7.56 ± 0.37a

5.46 ± 0.35b

1.0523

0.0002

Apoptosis of dermal papilla cells

 Early apoptotic ratio (Q4)

33.95 ± 0.40b

32.86 ± 1.04b

36.71 ± 0.67a

29.16 ± 0.47c

1.9470

< 0.0001

 Later apoptotic ratio (Q2)

33.46 ± 0.57b

32.15 ± 1.33b

36.11 ± 0.73a

31.40 ± 0.57b

2.4323

0.0032

 Total apoptosis ratio (Q4 + Q2)

67.41 ± 0.51b

65.01 ± 1.50b

72.83 ± 1.14a

60.56 ± 0.47 b

2.8310

< 0.0001

  1. Note: Data shown are mean values ± s. d., and n = 8 per group. In the same row, values with same letter superscripts mean no significant difference (P > 0.05), with different letter superscripts mean significant difference (P < 0.05)