Fig. 10

Experimental methods. a A freshly pupated pupa and a polylysine-coated glass plate. The scale bar (10 mm) is also applicable to the other panels. b A manipulated pupa. The forewing has been lifted, exposing the ventral forewing and the dorsal hindwing. c A manipulated pupa covered with a plastic film. The wing color pattern is emerging. d Another manipulated pupa covered with a plastic film. The wing color pattern is emerging. The forewing pattern is almost complete, but the hindwing pattern is not. e A manipulated pupa on a glass plate. f Another manipulated pupa on a glass plate. The ventral forewing and the dorsal hindwing are seen through the glass. Parts of the hindwing that did not contact the glass plate developed a thin cuticle layer. g A manipulated pupa on a gelatin-coated plate. h A manipulated pupa on a gelatin-coated plate. The ventral forewing and the dorsal hindwing are seen through the glass. i Another manipulated pupa on a gelatin-coated plate. The color pattern is almost complete. Parts of the hindwing that did not contact the gelatin-coated plate developed a thin cuticle layer. j A manipulated pupa on alumina file. k Semiparabiosis. Two pupae with lifted forewings are bound together. l A manipulated pupa in a no-contact configuration. Because of the height gap at the center of a glass-bottom dish, the dorsal hindwing made no physical contact