Table 3 Primers used to detect LoxP and Ywhah

Ywhah LoxP

Forward: 5′- TAATTGTGAGCCACCCGAAATGA − 3′

Reverse: 5′- GCCAACGACCAATGCCAATTATAG − 3′

WT: 226

Floxed: 292

Ywhah knockout

Forward: 5′- CCTGATCTAGGATAGCTAGGGCTACATAG − 3′

Reverse: 5′- AGTATACCTTTTGGAGACAGGATCTATTATAGCC − 3′

Deletion gives band at: 390

Ywhae LoxP

Forward: 5′- GCATGTGTTTGTCTGTCAGAGGAC − 3′

Reverse: 5′- AGGTACCAAAACAGTAAGCCATCTCCCTA − 3′

WT: 450

Floxed: 536

Ywhae knockout

Forward: 5′- TTCTTTTGTAGAAATTGGGGAAGGTCATGG − 3′

Reverse: 5′- AGGTACCAAAACAGTAAGCCATCTCCCTA − 3′

Deletion gives band at: 664

Generic Cre

Forward: 5′- GCG GTC TGG CAG TAA AAA CTA TC − 3′

Reverse: 5′- GTG AAA CAG CAT TGC TGT CAC TT − 3′

Cre Positive: 100