Fig. 1

Detection of mRNAs for Ywha isoforms in oocytes and eggs of two mouse strains. Messenger RNA was isolated from oocytes and eggs, reverse transcribed and prepared for PCR as indicated in Methods. The PCR reactions using isoform-specific Ywha primers were repeated twice for each isoform and the products were loaded in adjacent lanes. The upper panel (a) displays PCR products of extracts of oocytes and eggs from outbred ICR (CD-1) mice. The lower panel (b) displays PCR products of extracts of oocytes and eggs from inbred C57BL/6 J mice. H2afz served as a positive PCR control. In all cases, no bands were seen in the negative controls consisting of all the PCR reaction mixture components excluding the template. Message corresponding to six Ywha isoforms was detected in both strains. The signal for Sfn message was barely detectable in oocytes of ICR(CD-1) oocytes