Table 1 Primer Sequences for Qualitative Real-Time Polymerase Chain
Gene | Primer nucleotide sequence |
|---|---|
Shh | Forward: CTGACCGTGACCGTAGCAAGT Reverse: TGGATGTGGGCTTTGGACTCA |
Sox 9 | Forward: CTGACCGTGACCGTAGCAAGT Reverse: TGGATGTGGGCTTTGGACTCA |
ANCN | Forward: ATGGCTTCCACCAGTGCG Reverse: CGGATGCCGTAGGTTCTCA |
collagen II | Forward: GCTCCCAGAACATCACCTACCA Reverse: ATTCCTGCTCAGGCCCTCC |
collagen X | Forward: CCCTTCTGCTGCTAGTGTC Reverse: GTCTTGGTGTTGGGTTGT |
RUNX2 | Forward: CCTTCCACTCTCAGTAAGAAGA Reverse: TAAGTAAAGGTGGCTGGATAGT |
ALP | Forward: CCTCTTGGGTCTCTTTGAGC Reverse: CAATCCTGCCTCCTTCCA |
PPAR-γ | Forward: GCATCCCCACCCTACTATTCTG Reverse: GAGGGAGTTGGAAGGCTCTTC |
leptin | Forward: GTCGTCGGTTTGGACTTCATC Reverse: CGGAGGTTCTCCAGGTCGTTG |
Annexin V | Forward: GCAGAACTAACAGCCATAA Reverse: AGAACCACCAACATCCTC |
B2M | Forward: AACGTGGAACAGTCAGACC Reverse: AGTAATCTCGATCCCATTTC |