Fig. 1

Loss of Tet proteins does not impair pluripotency. a Schematic showing the generation of the Oct4:IRES:eGFP parental cell line followed by deletion of each TET protein individually or combinatorially (DKO = Tet1^−/−:Tet2^−/−, TKO = Tet1^−/−:Tet2^−/−:Tet3^−/−). b Representative histogram showing eGFP expression in a WT clone. Parental (GFP-) mESC was used as a negative control. c Brightfield and fluorescent micrographs (20x) of WT Oct4:IRES:eGFP before and after exposure to 5 μM retinoic acid to induce differentiation. d Representative western blot of Tet1,Tet2, and common pluripotency factors in each line. GAPDH was used as a loading control. e Representative histogram of eGFP expression in WT, Tet1^−/−, Tet2^−/−, DKO, and TKO clone. f RT-qPCR of endoderm (Gata6), trophectoderm (Cdx2), and mesoderm (Brachyury/T) associated transcription factors. * = p < .05 compared to WT.