Fig. 2

Identification and expression profile of vitellogenin (Vg) and vitellogenin receptor (VgR) of M. vitrata. a Vg on 10% SDS-PAGE. Vg protein was identified in 5 days old adult female hemolymph (HL) and ovary (OV), but not in male HL, testis (TE), or L5 larvae. L5 larval HL contained a large amount of storage protein (SP). b LC-MS/MS analysis of Vg protein band and its MASCOT prediction. The sequence of M. vitrata Vg (GenBank accession number: MG799570) contains fragments identified by LC-MS/MS. c Expression analyses of Vg and VgR in females at different ages (1–5 days after emergence) and males (5 days old after emergence) using RT-PCR (gel picture) and RT-qPCR (graph). All treatments in RT-qPCR were independently replicated three times. β-Actin expression was used as reference in RT-qPCR to normalize target gene expression level. Different letters above standard deviation bars indicate significant difference among means at Type I error = 0.05 (LSD test)