A dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryos

Table 1 Effect of low dose of PD0325901 in bovine embryos

Treatments	N	Total cells	ICM	TE	ICM:TE
DMSO	7	246 ± 24^b	78 ± 13^a	166 ± 14^b	0,47 ± 0,12 ^a
PD032 (0.4 μM)	4	282 ± 29^a	58 ± 10^b	224 ± 20^a	0,26 ± 0,07 ^b
PD032 (1 μM)	6	277 ± 27^a	49 ± 9^b	228 ± 20^a	0,21 ± 0,06 ^c
PD032 (2 μM)	9	270 ± 26^a	39 ± 7^c	230 ± 20^a	0,17 ± 0,05 ^d

Number of inner cell mass (ICM), trophectoderm (TE) and total cells in embryos cultured under different conditions. Results are from three biological replicates. “N” is the number of embryos analyzed. Values are mean ± S.E. estimated by a GLMM analysis with Poisson distribution and log as link function (for Total Cells, ICM and TE), and with Binomial distribution with logit as link function for (ICM:TE ratios). Different letters denote significant differences, p < 0.05

ISSN: 1471-213X