Fig. 4
From: A dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryos
Effect of high dose of MEK inhibitor on NANOG and SOX17 expression in day 8 IVF bovine embryos. a Immunostaining detection of NANOG and SOX17 in representative day 8 bovine IVF blastocysts. Each image is a single optical section. b Scatter plots showing NANOG and SOX17 positive cells from control and treatments. Data were analysed by Kruskal Wallis and data are presented as Median ± IQR. Different superscripts indicate significant difference between groups (p < 0.05). N: number of embryos counted after immunofluorescence detection, N = 9; 8; 12 and 11 for DMSO, 2.5 μM PD032, 5 and 10 μM PD032 groups, respectively. Scale bar: 10 μm