Fig. 6

Competition EMSA with CIRBP. (A) EMSA between TAMRA-labeled PMF 3′ UTR 99–368 RNA (30 ng; red) and rCIRBP/ECFP (1.5 μg; RBP; green), with competition using 100X (3 μg) of a specific competitor (SC; unlabeled PMF 3′ UTR 99–368) or a non-specific competitor (NC; unlabeled Keratin 3′ UTR 205–441). (B) EMSA with TAMRA-labeled PMF 3′ UTR 26–668 RNA (30 ng; red) and rCIRBP/ECFP (1.5 μg; RBP; green), with competition using 100X (3 μg) of four different unlabeled competitors: SC1 = PMF 3′ UTR 26–288, SC2 = PMF 3′ UTR 26–565, SC3 = PMF 3′ 26–668, or NC = Keratin 3′ UTR 205–441