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Table 3 Number of female spiders that were used to study the Vg at the protein level

From: Vitellogenins in the spider Parasteatoda tepidariorum – expression profile and putative hormonal regulation of vitellogenesis

Stage of tested development

Number of female spiders per sample

SDS-PAGE

ELISA

Ovaries

Midgut glands

Ovaries

Hemolymph

Penultimate nymphal stage

5

6

10

Last nymphal stage

3

3

3

6

Day that sexual maturity was reached

2

2

2

4

Three days after mating

2

1

2

3

Day of the first oviposition

1

1

3

  1. Number of P. tepidariorum females that were used to prepare one sample from the midgut glands, ovaries and hemolymph to determine the Vg at the protein level at various ages