Fig. 3

Level of vitellogenin (at transcript and protein levels) in response to 20-hydroxyecdysone administration. The profile of the vitellogenins [mean ± SD] of the P. tepidariorum females that were treated with a Ringer solution and 10, 100 and 200 ng of 20E per individual. Vg results in the midgut glands at the transcript (a.1) and protein levels (a.2), the ovaries at the transcript (b.1) and protein levels (b.2) and the hemolymph at the protein level (c). The dashed line box shows the physiological time of vitellogenesis. Lowercase letters indicate significant differences between experimental groups at the same time point (Tukey’s multiple comparisons test, p ≤ 0.05)