Interleukin-6 increases inner cell mass numbers in bovine embryos

Table 1 Cleavage and blastocyst formation across each study

Study	Treatment	Treatment dates(s)	IVF replicates	Total embryos	Cleavage*	Day 7 blastocyst*	Day 8 blastocyst*
A	0 ng/ml IL6	Day 5	4	317	N/A	12.8 ± 1.3^a	21.8 ± 3^a
	1 ng/ml IL6	Day 5		316		10.8 ± 1.4^a	21.8 ± 3.7^a
	10 ng/ml IL6	Day 5		323		16.8 ± 3.3^a	23.7 ± 4.6^a
	100 ng/ml IL6	Day 5		320		12.7 ± 4.2^a	23.7 ± 6.3^a
B	0 ng/ml IL6	Day 3	6	240	N/A	10.8 ± 4^a	24.2 ± 6.3^a
	100 ng/ml IL6	Day 3		240		14.6 ± 3.4^a	30.4 ± 6.8^a
	100 ng/ml IL6	Day 5		240		13.8 ± 3.3^a	25.8 ± 6.9^a
	100 + 100 ng/ml IL6	Day 3 + 5		240		12.5 ± 4.8^a	32.9 ± 6.4^a†
C	0 ng/ml IL6	Day 1	4	249	73.4 ± 3.7^a	12.3 ± 4.2^a	20.1 ± 3.5^a
	1 ng/ml IL6	Day 1		297	80.1 ± 5.9^a†	17.9 ± 2.5^a	27.1 ± 2.4^a
	10 ng/ml IL6	Day 1		301	76.4 ± 5.6^a	18.1 ± 3.7^a	27.7 ± 4.7^a
	100 ng/ml IL6	Day 1		248	76.4 ± 3.6^a	19.4 ± 4.1^a	29.6 ± 4.2^a
D	0 ng/ml IL6	Day 1	6	395	74.8 ± 4.5^a	11.5 ± 1 ^a	20.3 ± 2.1 ^a
	100 ng/ml IL6	Day 1		437	76.8 ± 2.2^a	10.8 ± 1.7 ^a	21.5 ± 2.5 ^a
	100 ng/ml IL6	Day 5		392	77.8 ± 3.8^a	10.9 ± 1 ^a	21.6 ± 2.4 ^a
	100 + 100 ng/ml IL6	Day 1 + 5		440	75.2 ± 5.2^a	10.2 ± 1.8 ^a	19.4 ± 3.7 ^a
	200 ng/ml IL6	Day 1		418	76.7 ± 3.7^a	9.8 ± 1.6 ^a	18.9 ± 1.7 ^a
E	0 ng/ml IL6	Day 5	4	347	87.7 ± 0.1^a	15.8 ± 0.1^a	27.9 ± 0.1^a
	100 ng/ml IL6	Day 5		337	85.3 ± 0.1^a	17.4 ± 0.1^a	26.1 ± 0.1^a
	200 ng/ml IL6	Day 5		392	83.9 ± 0.1^a	20.5 ± 0.1^a	30.4 ± 0.1^a
	100 + 100 ng/ml IL6	Day 1 + 5		357	81.1 ± 0.1^a	18.9 ± 0.1^a	29.1 ± 0.1^a
F	0 ng/ml IL6 (Group)	Day 1	4	175	138/175^a	17/138^a	29/138^a
	Individual
	0 ng/ml	Day 1		100	65/100^b	0/65^b	0/65^b
	100 + 100 ng/ml IL6	Day 1 + 4		100	79/100^a	0/79^b	7/79^c
	200 ng/ml IL6	Day 1		100	73/100^ab	0/73^b	7/73^c

Different superscripts within each study denote differences. Significance established at P < 0.05
† indicates a trend (P = 0.06 in study B and P = 0.09 in Study C) when compared with the control)
*Data for studies A-E are presented as the Mean% ± SEM. Data from study F are presented as proportions

ISSN: 1471-213X