Fig. 1
Emergence time under constant conditions and LD 12:12. (a-c) Box plots show the median time to emergence for each vial and strain (n > 8 vials; 30 eggs/vial) under three regimes. Whiskers extend up to highest and lowest values and dots show the individual data. Under (a) constant darkness (DD) at 25 °C and (b) constant light (LL) at 25 °C asterisks show that perl differs significantly from other strains (p < 0.05) based on Kruskal-Wallis test for multiple independent samples. (c) LD 12:12 (T24) at 25 °C. (d) Adult-emergence profiles of the three strains (n = 10 vials; 300 eggs/vial) under T24 where percentage emergence is plotted against zeitgeber time, 0 being the time of lights-on. Shaded region represents duration of the LD cycle during which lights were off. (e) Box plots show period values obtained by COSINOR analysis for each strain under T24. Whiskers extend up to highest and lowest values and dots show the individual vial median data points. Black line depicts the period of T-cycle