Fig. 3

Knockdown of dCBP expression in the Drosophila nervous system decreased H3K23ac levels and led to a defect in courtship learning ability. a The RNAi efficiencies of CBP in larval brain-discs was detected by RT-qPCR. Female UAS-GFP RNAi (control line), UAS-dCBP RNAi flies were crossed with Elav-GAL4 males. rp49 was used as the reference gene. Error bars represent standard error of the mean. b Salivary gland immunostaining was performed in dCBP RNAi flies with anti-H3K23ac. RNAi induced knockdown was driven by SG-GAL4. c Western blotting analysis of H3K23ac protein levels from whole-salivary gland lysates after the depletion of dCBP. d Western blot for H3K23ac revealed decreased H3K23ac expression in the brain lysates of dCBP RNAi flies. The quantified data was measured by the software Image J. N=3 biological replicates. e Courtship learning analyses of dCBP RNAi flies. Compared to GFP RNAi flies, the dCBP-knockdown flies exhibited learning defects. f dCBP RNAi exhibited normal courtship memory. dCBP knockdown in the mushroom body of adult flies was driven by Ok107-GAL4. Unpaired t-test was used for statistics. Error bars represent the standard error of the mean; the number of samples was indicated in the bar. n.s., not significant. ***p<0.001