Fig. 6

Dα7 mediates the effect of nicotine on eclosion but not on larval brain size. a, b wB flies were reared on control (black bars) or 0.3 mg/ml nicotine (red bars) food and the number of eclosed flies was determined. a wB flies were reared in control food or food with nAChRs blockers: 10 nM (light blue bars) or 100 nM (dark blue bars) α-bungarotoxin (α-B) or 100μM (purple bars) mecamylamine (Mec); these drugs significantly decreased eclosion. b Blocking nAChRs during developmental nicotine did not revert the effect of nicotine. c-e Dα7^WT (dark grey bars) or Dα7^mut (light grey bars) flies were reared on control or 0.1 mg/ml nicotine food and the number of eclosed flies or brain size was determined. c Dα7 mediates the effects of developmental nicotine exposure on eclosion. d, e Dα7 does not regulate larval or adult brain size. a, b wB flies. Sample size: n = 14 vials, control food; n = 18, 0.3 mg/ml nicotine food; n = 12 vials each for 10 nM or 100 nM α-bungarotoxin, 100μM mecamylamine,100 nM α-bungarotoxin+ 0.3 mg/ml nicotine, 100μM mecamylamine + 0.3 mg/ml nicotine; n = 10 vials, 10 nM α-bungarotoxin+ 0.3 mg/ml nicotine; 2 independent experiments for nAChR blockers, 3 for control and nicotine food. c Sample size: n = 12 vials for Dα7^WT and Dα7^mut, control food; n = 18, Dα7^WT nicotine food; n = 16, Dα7^mut nicotine food from 3 independent experiments. d Sample size: n = 10 larval brain hemispheres Dα7^WT, control food; n = 10, 0.1/mg/ml nicotine food; n = 6 Dα7^mut, control food; n = 11, 0.1 mg/ml nicotine food from 3 independent experiments, except Dα7^mut in control food, single experiment. e Sample size: n = 6 brains, Dα7^WT control food; n = 16, 0.1/mg/ml nicotine food; n = 7 Dα7^mut, control food; n = 4, 0.1 mg/ml nicotine food from 2 independent experiments. a-e Kruskal-Wallis test, followed by pairwise comparisons adjusted for multiple comparisons