Fig. 3From: Developmental nicotine exposure affects larval brain size and the adult dopaminergic system of Drosophila melanogasterDevelopmental nicotine exposure decreases TH fluorescence in adult brains. Flies were raised on control food or food laced with 0.3 mg/ml nicotine. Larvae were dissected at the 3rd instar stage of development; adults were dissected 4 days after eclosion. The brains were stained with an antibody against tyrosine hydroxylase (TH). a Corrected TH brain hemisphere fluorescence, which normalized the staining fluorescence to background levels, showed that developmental nicotine exposure had no statistically significant effect on TH staining in larval brains. b Corrected TH central brain fluorescence was significantly decreased in adult brains of flies exposed to nicotine during development. Sample size was n = 22 brain hemispheres for control and n = 18 brain hemispheres for nicotine from 5 independent experiments for the larval stage, and n = 10 brains for control and n = 17 for nicotine from 5 independent experiments for adult. Mann-Whitney U-test (a) and Student’s t-test (b) were used to compare the control versus the nicotine conditionBack to article page