Molecular signatures of epithelial oviduct cells of a laying hen (Gallus gallus domesticus) and quail (Coturnix japonica)

Table 3 Expression of the oviduct, epithelial, and progenitor markers in oviduct tissue and cultured oviduct epithelial cells in hen and quail

Gene panel	Gene	Hen		Quail
Gene panel	Gene	Tissue^a	Cell culture^b	Tissue^a	Cell culture^b
Oviduct markers	ESR1	+	+	+	+
	OVAL	+	+	+	ND
	OVM	+	+	+	ND
Epithelial markers	KRT5	+	+	+	+
	KRT14	+	+	+	+
	OCLN	+	+	+	+
Stem-like/Progenitor markers	CD44	+	+	+	+
	LGR5	ND	+	+	+
	MSI1	+	+	+	+
	SOX9	+	+	+	+
	NANOG	+	+	+	+
	OCT4/cPOUV	ND	+	+	+

^aHen/quail oviduct tissue, divided into three fragments: INF infundibulum, DM distal magnum, and PM proximal magnum; ^bHen/quail oviduct epithelial cell culture derived from different parts of the oviduct (INF, DM, or PM) and cultured in vitro; “+” denotes positive result of RT-qPCR analysis (Ct < 35), meaning that the gene was expressed in a given sample. ND not detected

ISSN: 1471-213X