Fig. 4

pum1 is not a PGC marker in O. dioica. In situ hybridization with a DIG-labeled RNA probe showed that pum1 transcripts remain localized to the surface of the developing embryos from gastrulation stage (panels a-c). In situ labeling with a fluorescent-labeled RNA probe revealed detection of pum1 signal outside of the embryos prior to hatching (panels d-f). Nuclei (red signal) were visualized by DAPI staining. The developmental stages included are: late gastrulation stage (a), early tailbud (2.5 h post-fertilization) (b), and close to hatching (c) - (f). The white triangle points to the pum1 signal. Scale bar: 50 μm