Fig. 5
From: Expression and function of microRNA-9 in the mid-hindbrain area of embryonic chick
MiR-9 reduced size of midbrain. Broad overexpression of miR-9 along the DV axis of the midbrain result often in smaller midbrains (15/18). Transfection of pSil-miR-9 (a-c) in right brain halves at HH8 (a), HH9 (b), HH10 (c) reduced the size of midbrain halves compared to left control side. (d-f): Open-book preparation of midbrain with ectopic miR-9 duplex from HH8 shows a reduced growth of the electroporated side (d-f) and a weakened expression of EN1 (e). 3A10 immunostaining showed a relatively normal pattern of neurogenesis only compressed into a smaller area (f). Abbreviations: FP-floor plate, Mes-mesencephalon, MHB-mid-hindbrain boundary, Rh-rhombencephalon, RP-roof plate