Fig. 5
From: Unique N-terminal sequences in two Runx1 isoforms are dispensable for Runx1 function
Restored basophil development in Runx1 ΔP1:P2TAA /ΔP1:P2TAA mice. a Dot plots showing CD4/CD8 expression in total thymocytes (upper) and lymph node T cells (lower) of 4 to 6 week-old Runx1 P1N/P1N and Runx1 ΔP1:P2TAA: ΔP1:P2TAA mice. b Graph showing CD4+ T cells to CD8+ T cells in lymph nodes of Runx1 +/+ (lane 1), Runx1 P1N/P1N (lane 2) and Runx1 ΔP1:P2TAA: ΔP1:P2TAA mice (lane 3) mice. Mean ± SD. * P < 0.05. ** P < 0.01. c Flow cytometry analysis of basophil differentiation. Dot plots showing expression of IgE, CD49b and FcεRI in bone marrow cells from Runx1 +/+, Runx1 P1N/P1N and Runx1 ΔP1:P2TAA: ΔP1:P2TAA mice. d Graphs showing numbers of CD49b+IgE+ cells in bone marrow from Runx1 +/+ (lane 1), Runx1 P1N/P1N (lane 2) and Runx1 ΔP1:P2TAA: ΔP1:P2TAA mice (lane 3) mice. Mean ± SD. * P < 0.05, **P < 0.01. e Semi-quantitative RT-PCR analysis of Mcpt8 transcript, a molecular marker of basophils, in bone marrow cells from mice of indicated genotypes. One representative of two experiments