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Table 1 Viability of TgTP6.4 hemizygotes and homozygotes assessed by frequencies of tauGFP-positive mice produced in different crosses

From: Comparison of two related lines of tauGFP transgenic mice designed for lineage tracing

 

Genetic cross (female × male)

Progeny

Cross 1

(experimental cross)

Cross 2

(control cross)

Cross 3

Tg/− × Tg/−

Tg/− × −/−

−/− × Tg/−

A. Mice classified for tauGFP at ~3 weeks

 Total born

336

140

348

 Dead before weaning

52 (15.5%)a

3 (2.1%)

8 (2.3%)

 Dead after weaning (most unclassified)

5 (1.5%)

0

3 (0.9%)

 Viable at weaning

284

137

340

 TauGFP not classified

46

6

139

 TauGFP classified

238

131

201

 TauGFP-positive

133 (55.9%)b,c

57 (43.5%)d

79 (39.3%)e

 TauGFP-negative

105 (44.1%)

74 (56.5%)

122 (60.7%)

 Pre-weaning death attributable to crossj

13.6%

NA

NA

B. Fetuses classified for tauGFP at E14.5

 Total conceptuses

193

192

 

 Moles (early deaths)

16 (8.3%)f

9 (4.7%)

 

 Dead fetuses

2 (1.0%)f

2 (1.0%)

 

 Total live fetuses

175

181

 

 TauGFP-positive

129 (73.7%)g,h

85 (47.0%)i

 

 TauGFP-negative

46 (26.3%)

96 (53.0%)

 

 Death attributable to crossk

3.8%

NA

 
  1. Abbreviations: −/− non-transgenic TgTP6.4 −/− was wild-type, (C57BL × CBA/Ca)F1, Tg/− hemizygous TgTP6.4 Tg/−, NA not applicable.
  2. In (A), data were compiled retrospectively from breeding records and not all offspring were classified. Data were excluded if the whole litter died between birth and weaning or no offspring were classified. In later litters it was recorded that tauGFP expression was mosaic in ear tissue of some tauGFP-positive offspring. aThe frequency of death before weaning was significantly higher in cross 1 than cross 2 (P < 0.0001 by Fisher’s exact test). b-eThe proportion of tauGFP-positive progeny in cross 1 differed significantly by goodness of fit chi-square test from 75% expected, if all homozygotes and hemizygotes are viable (P < 0.0001)b, and from 67% expected, if all homozygotes die but all hemizygotes are viable (P = 0.0005)c. The proportion of tauGFP-positive progeny did not differ significantly from 50% expected in cross 2 (P = 0.1615 by goodness of fit chi-square test)d but it did differ from this expected proportion in cross 3 (P = 0.003)e. f In (B), the frequencies of moles plus dead fetuses did not differ significantly between crosses 1 and 2 (P = 0.2463 by Fisher’s exact test). g-i The proportion of tauGFP-positive fetuses in cross 1 did not differ significantly by goodness of fit chi-square test from either 75% expected, if all homozygotes and hemizygotes are viable (P = 0.7642)g or from 67% expected, if all homozygotes die but all hemizygotes are viable (P = 0.0578)h. In cross 2, the proportion of tauGFP-positive progeny did not differ significantly from 50% expected (P = 0.4543 by goodness of fit chi-square test)i
  3. The percentage death, attributable to production of TgTP6.4 Tg/Tg homozygotes in cross 1, was calculated by correcting for sporadic death in cross 2 [61]:
  4. j \( \left[1-\left(\frac{\mathrm{viable}\ \mathrm{mice}\ \mathrm{weaned}\ \mathrm{in}\ \mathrm{experimental}\ \mathrm{cross}\ 1}{\mathrm{total}\ \mathrm{born}\ \mathrm{in}\ \mathrm{experimental}\ \mathrm{cross}\ 1}/\frac{\mathrm{viable}\ \mathrm{mice}\ \mathrm{weaned}\ \mathrm{in}\ \mathrm{control}\ \mathrm{cross}\ 2}{\mathrm{total}\ \mathrm{born}\ \mathrm{in}\ \mathrm{control}\ \mathrm{cross}\ 2}\right)\right]\times 100 \)
  5. k \( \left[1-\left(\frac{\mathrm{live}\ \mathrm{fetuses}\ \mathrm{in}\ \mathrm{experimental}\ \mathrm{cross}\ 1}{\mathrm{total}\ \mathrm{conceptuses}\ \mathrm{in}\ \mathrm{experimental}\ \mathrm{cross}\ 1}/\frac{\mathrm{live}\ \mathrm{fetuses}\ \mathrm{in}\ \mathrm{control}\ \mathrm{cross}\ 2}{\mathrm{total}\ \mathrm{conceptuses}\ \mathrm{in}\ \mathrm{control}\ \mathrm{cross}\ 2}\right)\right]\times 100 \)
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BMC Developmental Biology

ISSN: 1471-213X

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