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Fig. 2 | BMC Developmental Biology

Fig. 2

From: Generation and characterization of a novel transgenic mouse harboring conditional nuclear factor-kappa B/RelA knockout alleles

Fig. 2

Confirmation of PGKneobpA cassette removal. The internal probes hybridized to Asp718-digested genomic DNA detect 18.7-kb WT bands, 3.7-kb targeted (floxed-neo) allele bands, and 2.0-kb CKO allele bands. Tail DNA was isolated from pups generated by mosaic females (RelA floxed-neo/+ ↔ RelA CKO/+; Gt(ROSA)26Sor FLP1/+) and a wild-type C57BL/6J male, and digested by Asp718. The blot was hybridized with the internal probe 1. When the same filter was stripped and rehybridized with the 3′ probe, all CKO/+ and neo/+ samples showed 4.1-kb mutant bands and 4.9-kb WT bands (data not shown)

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BMC Developmental Biology

ISSN: 1471-213X

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