Fig. 3

Different BRE::gfp transcriptional activity patterns in the postnatal vasculature. a Overview of a P4 retina with immunodetection of Endomucin and GFP. Boxed areas show the sprouting front (b) and the centre (c) of the retinal plexus. Single staining for GFP is shown in the bottom panels. Asterisks depict GFP-positive tip cells, the arrows point to the veins, while the arrowheads show the arteries. d–g The retina at P8, immunostained for Endomucin and GFP, has a multi-layered vascular plexus consisting of the vascular border (d) and centre (e) of the primary plexus, the perpendicular vessels (f) and outer plexus (g). Single GFP staining is shown in the lower panels. Scale bars: 500 μm (a); 75 μm (b–g)