Fig. 4

HH signalling blockade prevents the induction of GLI2 target and of cardiac progenitor-enriched genes. a qPCR analysis of mRNA expression of Ptch1 and indicated cardiac progenitor-enriched genes in day 0 mES cells (white bars) or in cultures kept in differentiation condition for 7 days, in the presence of KAAD-cyclopamine (black bars) or vehicle (methanol, grey bars). Error bars represent +/- SEM; n = 3 ( ) p < 0.05 by one-tailed paired T-test. b Immunofluorescence detection of myosin heavy chain (red channel) in similar day 7 cultures treated with vehicle or KAAD-cyclopamine. DNA was counterstained with Hoechst (blue channel). c MyHC^+ve cells from (b) were counted. Error bars represent +/- SEM; n = 3 biological replicates