Fig. 2

Overexpression of GLI2 in mES cells enhances transcript expression of a GLI2 target and of cardiac progenitor-enriched genes. qPCR analysis of mRNA expression of indicated genes known to be active in HH-responsive cells, mesoderm/precardiac mesoderm cells, and cardiac progenitor cells, in mES[Ctrl] (white bars) and mES[Gli2] (grey bars) cultures on the days noted in the figure. Expression levels were normalized to β-actin, calibrated to day 0 mES[Ctrl] culture expression levels, and presented as a percentage of the highest expression level recorded, per gene. Error bars represent +/- SEM. The number of biological replicates analyzed (n) is indicated on each graph. One-tailed Student’s T-tests were used for statistical analyses. Grey lines represent paired T-tests; black lines represent unpaired T-tests; ( ) p < 0.05