Fig. 7

Continuous EdU treatment after partial amputation. a Schematic drawing of the tail plate with indication of the amputation level. b Number of EdU-positive and -negative adhesive gland cells after 96 h of regeneration on the uncut side and cut side. The numbers are based on the average of 12 specimen, error bars indicate the standard deviation. c-d Representative images of PNA, EdU, and Dapi staining after 96 h of regeneration. Note the higher number of EdU positive cells on the side of amputation. Images in (c) are maximal intensity projections of optical sections and in (d) one single optical section is shown. The arrowhead indicates an EdU-positive adhesive gland cell, the arrow highlights an EdU-negative adhesive gland cell. Scale bars: (C) 20 μm, (D) 10 μm