Fig. 4

Regeneration of adhesive organs after tail plate amputation. a Schematic drawing of Macrostomum lignano with indication of the amputation level. b-e PNA lectin and Macif1 double staining of regenerating specimen after (b1-3) 48 h, (c1-3) 60 h, (d1-3) 72 h, and (e1-3) 96 h of tail plate regeneration. Images are Z projections of confocal stacks. Note that according to Egger et al. [9] the early regeneration blastema is slightly bend towards the ventral side. Therefore, in figures B1-C3 the location of the differentiated anchor cells appears to be within the tail plate. Dotted line indicates the outline of the blastema. Scale bars: 20 μm