Fig. 2

Daf1+ DE cells have lower cell-matrix adhesion than Daf1- DE cells. a A schematic drawing showing experimental design. The sorted DE cells were plated onto matrigel-pre-coated dishes and cultured for 24 h (b, c) or 90 min (d, e). b Transmission images of the cells derived from the Daf1+/-DE cells after cultured for 24 h. c After 24 h culture, the Daf1 + DE cells gave rise to fewer cells. d Cell-matrix adhesion assay, after 90 min culture. Quantification of the attached cells. The adhesion ability of Daf1 + DE cells onto matrigel is weaker than that of the Daf1-DE cells. e Quantification of Cleaved caspase3/7+ apoptotic cells. The Daf1 + DE cells gave rise to a larger proportion of apoptotic cells. f The Integrin expression profiles of the DE cells detected by RT-PCR analysis. Student’s two tailed t-test. Scale bars; 100 μm