Fig. 1
From: β-catenin is required in the neural crest and mesencephalon for pituitary gland organogenesis
a–b Rosa mT/mG reporter mice enable fluorescent detection of cre recombinase activity, where green fluorescence indicates areas of cre recombination and red fluorescence indicates areas with no recombination. a Wnt1-cre; Rosa mT/mG e8.5 embryo. Arrow indicates recombinase activity in the midbrain. b P0-cre; Rosa mT/mG e8.5 embryo. c–h X-gal staining (blue) reveals cre recombinase activity in Rosa stopLacZ reporter mice. c–f and h sagittal sections counterstained with neutral red. c Mid-sagittal section of an e12.5 Wnt1-cre; Rosa stopLacZembryo. Dotted lines indicate the midbrain (M) boundaries with the diencephalon and hindbrain. The boxed area is magnified in d. d Boxed area indicated in c. Arrow indicates Rathke’s pouch; R indicates rostral head mesenchyme; C indicates caudal head mesenchyme e e12.5 Wnt1-cre; Rosa stopLacZ section at the lateral extreme of Rathke’s pouch (arrow). f e14.5 Wnt1-cre; Rosa stopLacZ. Arrow indicates X-gal stained cells within the pituitary anterior lobe. g Ventral view of a dissected e18.5 Wnt1-cre; Rosa stopLacZ pituitary. h Mid-sagittal section of an e12.5 P0-cre; Rosa stopLacZ embryo. Scale bars in a, b, and d–h equal 100 μm. Scale bar in c equals 1 mm