Fig. 6

RT-PCR analysis confirms differential expression of genes in Dop1R2 RNAi flies. a Transcript levels assessed by RT-PCR. RNA obtained from Dop1R2 RNAi (genotype: w¹¹¹⁸;UAS-dsDop1R2/+;Act5C-GAL4/+) and control pupae (genotype: w¹¹¹⁸;UAS-dsDop1R2/+;TM6B/+) was reverse transcribed, and PCR was performed in triplicate using primer sets corresponding to gene of interest or to Act5C (as a normalization control). b Quantification of transcript levels. The average band intensity of Dop1R2 RNAi PCR products was normalized to control PCR products for Act5C. Error bars indicate the Standard Error of the Mean (SEM), and significance was determined by comparing the difference in intensities of the RNAi PCR bands versus the control PCR bands using an unpaired t-test. * p < 0.05, ** p ≤ 0.01, *** p ≤ 0.001. Driver stock: Act5C-GAL4 (FBst0003954)