Fig. 3

Ubiquitous knockdown of Dop1R2 results in reduced adult emergence and wing and/or melanization phenotypes. a Ubiquitous knockdown of Dop1R2 at 29 °C results in 100 % of Dop1R2 RNAi flies (genotype: w¹¹¹⁸;UAS-dsDop1R2/+;Act5C-GAL4/+) failing to emerge, compared to control flies (genotype: w¹¹¹⁸;UAS-dsDop1R2/+;TM6B/+). At 25 °C, 19.8–72.1 % (line 1) and 53.1–78.1 % (line 2) of Dop1R2 RNAi flies develop into adults (‘escapers’). 25 °C n = 1580 and 29 °C n = 449. Line 1: 4 replicates at 25 °C and 5 replicates 29 °C. Line 2: 3 replicates at 25 °C and 29 °C. Error bars indicate the Standard Error of the Mean (SEM). b Escaper flies may exhibit two other phenotypes: hypomelanization and curly wing. Hypomelanization phenotype appears as reduced melanization of abdominal cuticle (arrows). c Curly wing phenotype appears as bent/curved adult wing (arrows). Driver stock: Act5C-GAL4 (FBst0003954)