Fig. 3
From: Mediator subunit Med12 contributes to the maintenance of neural stem cell identity
Expression of Med12-regulated cell adhesion genes increases during neuronal differentiation of mNS-5 NSCs. mNS-5 NSCs were seeded onto laminin-coated plates prior to initiation of neuronal differentiation by sequential withdrawal of growth factors as indicated in the schematic and described in Methods. RNA isolated from cells on 0, 2, 5, 8, and 11 days after initiation of neuronal differentiation was subjected to RT-qPCR. mRNA levels for each gene were normalized to β-actin mRNA and expressed relative to their corresponding mRNA levels on day 0 (D0′) of the differentiation protocol. Data represent the mean +/− SEM of three independent experiments performed in triplicate. Asterisks denote statistically significant differences in the relative mRNA levels for each gene compared to their corresponding levels on D0′ (Student’s t test, **p < 0.05, ***p < 0.01)