Fig. 6

Different morphological changes during muscle cell death. a-c The top panels show muscle fibers labelled with tau-GFP (green) and outlined dotted lines. The bottom panels show the myonuclei labelled with histone-mKO (white). a A doomed DEOM2 expressing a control shRNA (see Fig. 3a, ‘*’) disintegrates into fluorescently labelled vesicular fragments or sarcolytes. Nuclei become brighter as they condense. b A DIOM1 expressing Rm62-shRNA expressing (Fig. 2c, arrow) undergoes premature histolysis similar to the PCD seen in the control DEOM2. c Cell death of Cp1-shRNA expressing muscles involves shrinking of cells and depletion of tau-GFP (compare +60 h and + 67 h). Subsequently, discrete nuclear signals at +67 h become fuzzy and weak at +72 h