Fig. 1

Bioinformatics analysis of PfSMAD4. Phylogenetic analysis of the SMAD4 family. The phylogenetic tree was constructed by MEGA 5.0 using the neighbor-joining method with 1000 bootstrap replicates. The number shown at each branch indicates the bootstrap value (%). Percentages refer to identity values. The left frame showed the identities of the whole PfSMAD4 sequence with its orthologs. The middle frame: MH1 domain; the right frame: MH2 domain. These SMAD4 amino acid sequences using in the alignment and phylogenetic analysis are from HsSMAD4 (Homo sapiens, AAH02379.1), MmSMAD4-a (Mus musculus, EDL09559.1), MmSMAD4-b (Mus musculus, EDL09560.1), MmSMAD4-c (Mus musculus, EDL09561.1), ScSMAD4-1 (Serinus canaria, XP_009098382.1), ScSMAD4-2 (Serinus canaria, XP_009098384.1), CmSMAD4-a (Chelonia mydas, XP_007063905.1), DrSMAD4-a (Danio rerio, NP_001116172.1), XlSMAD4-1 (Xenopus laevis, NP_001090536.1), XlSMAD4-2 (Xenopus laevis, NP_001084387.1), BfSMAD4 (Branchiostoma floridae, AEU03847.1), SkSMAD4 (Saccoglossus kowalevskii, XP_002740765.2), SpSMAD4 (Strongylocentrotus purpuratus, XP_780740.3), CiSMAD4 (Ciona intestinalis, NP_001071944.1), NvSMAD4 (Nematostella vectensis, EDO31382.1), AaSMAD1/5 (Aedes aegypti, XP_001664103.1), DmMedea-a (Drosophila melanogaster, AAF57172.1), DmMedea-b (Drosophila melanogaster, AAN14277.1), DmMedea-c (Drosophila melanogaster, AAN14278.2), CgSMAD4 (Crassostrea gigas, EKC24133.1), PfSMAD4 (Pinctada fucata, AGY49100.1), SmSMAD4 (Schistosoma mansoni, XP_002574840.1), HrSMAD4 (Helobdella robusta, ESN93792.1), MlSMAD1/5 (Mnemiopsis leidyi, AEP16392.1), AsSMAD4 (Ascaris suum, ERG79533.1), TaSMAD4 (Trichoplax adhaerens, XP_002116214.1) and AqSMAD4 (Amphimedon queenslandica, XP_003388571.1)