Fig. 4

Combined effect of EGF and PLO on mPdx1 transduction. Two days and 7 days after mPdx1 adenoviral transduction of p2 hAECs grown in the presence of EGF and PLO, the expression of human pancreatic marker genes was assayed by means of qPCR. Expression was compared to cells that were cultured only in the presence of EGF. mRNA expressions were normalized to endogenous GAPDH expression. Similar to transduced cells cultured with EGF alone, the 7 genes- NKX2.2, PAX6, PDX1, RFX6, SOX17, CFTR and FOXA2—showed a dramatic and higher change in expression upon mPdx1 transduction. In most cases, expression of genes in cells grown on PLO was higher than their non-PLO counterpart. (* p < 0.05, ** p < 0.005, *** p < 0.0005; n = 2)