Fig. 1

Characterization of the actin cytoskeleton and extracellular matrix (ECM) during regeneration. a A DIC image of a late bud blastema transverse section indicating where the sagittal sections in (b, d) are located along the proximal/distal axis. b Phalloidin-rhodamine staining of sagittal sections through an EB, or from the Apical to basal regions of the LB. c Quantification of the organization of the actin cytoskeleton in images in (b). Error bars are the SEM, and students t-test was used to determine statistically relevant changes in organization (p < 0.002). d Immunodetection of the ECM molecule tenascin (Green) in EB, or apical to basal regions of the LB