Fig. 4

Meis2 is expressed in migrating NCC. (a) Meis2 expression in the neural plate stage at E8.5 becomes weak towards the neural plate border (arrows). Right: schematic embryo at E8.5 with a depicted section plane (dashed). (b-b”) Sections from rostral (b‘) to caudal (b‘’) of wild-type embryos at E9.5 revealing Meis2 in pharyngeal arches (PA), hindbrain (H) and in the cardiac outflow tract (OFT). (c-c”) Immunofluorescent double-labeling of Meis2 (green) and Tfap2 (red) in the region approximately depicted by a black rectangle in A’. (d-d”’) Transverse sections from rostral to caudal at E10.5 with immunostained Meis2. Sections levels correspond to the illustrated cartoon. Meis2 was detected in the hindbrain (H), forebrain (F), optic cup (OC), trigeminal nerve ganglion (V), otic vesicle (OV), facial nerve ganglion (VII), maxillary (Mx) and mandibular (Mb) process of PA1, in PA2 and outflow tract (OFT). (e) Immunofluorescence of Meis2 (green) and Twist1 (red) shows their co-expression in two areas labelled with rectangles in D”’. Upper panels (1) belong to the lateral zone (1) and lower panels (2) to the midline area (2)