Table 2 Examples of secondary antibodies used successfully with 3D-SIM
From: 3D structured illumination microscopy of mammalian embryos and spermatozoa
Host | Target | Fluorophore | Excitation [nm] | Dilution | Company | ID |
|---|---|---|---|---|---|---|
Donkey | Mouse IgG (H + L) | Alexa | 488 | 1:400 | Invitrogen | A-21202 |
Donkey | Mouse IgG (H + L) | Alexa | 594 | 1:500 | Invitrogen | A-21203 |
Donkey | Goat IgG (H + L) | Alexa | 488 | 1:400 | Invitrogen | A-11055 |
Donkey | Goat IgG (H + L) preadsorbed | Alexa | 594 | 1:400 | Abcam | ab150136 |
Donkey | Rabbit IgG (H + L) | Dylight (Alexa bleaches less) | 488 | 1:300 | Jackson Immuno Research | 711-485-152 |
Donkey | Rabbit IgG (H + L) | Dylight (Alexa bleaches less) | 594 | 1:500 | Jackson Immuno Research | 711-505-152 |
Goat | Mouse IgG (H + L) preadsorbed | Alexa | 594 | 1:300 | Invitrogen | A-11032 |
Donkey | Rat IgG (H + L) | Alexa | 594 | 1:300 | Invitrogen | A-21209 |