Fig. 7
From: 3D structured illumination microscopy of mammalian embryos and spermatozoa
Examples of 3D-SIM and confocal microscopy scans. a. 3D-SIM scans of a nucleus in a trophectoderm blastomere from a bovine blastocyst. b. 3D-SIM scans of a nucleus in a blastomere from a rabbit 21-cell embryo. c. 3D-SIM scans of a nucleus in a blastomere from a mouse morula. The background may have increased slightly since this morula was stored for 2 years in PBS under mineral oil between fixation and staining. d. 3D-SIM scans of a rabbit spermatozoon. e. Confocal microscopy scans of a nucleus in a blastomere from a rabbit 2-cell stage embryo. a1–e1. DAPI. a2–e2. NUP153. a3–e3. Lamin B. a4–e4. Composite of DAPI (grey), NUP153 (green) and lamin B (red). a5–d5. DAPI without a correction of the background signals. a6–d6. NUP153 without a correction of the background signals. a7–d7. Lamin B without a correction of the background signals