Fig. 9
From: A loss-of-function and H2B-Venus transcriptional reporter allele for Gata6 in mice
Expression of Venus at somite stages. a Frontal and lateral views of wholemount Gata6 H2B-Venus/+ embryos merged with brightfield images at E8.25 (4 somite stage, ss). Venus is expressed in the heart, sinus venosus, and gut endoderm. b Lateral views of wholemount Venus expression at E9.5 (23ss) in Gata6 H2B-Venus/+ embryos. c Immunofluorescence for GATA6 (red) on transverse sections through a Gata6 H2B-Venus/+ embryo. Varying levels of GATA6 co-expression with Venus (green) can be seen in the gut endoderm and heart. Some tissues express GATA6 exclusively, for example the body wall and endocardium, while other tissues express Venus only, such as the yolk sac and weak expression in the pharyngeal mesoderm. Nuclei are stained with Hoechst (blue)