Fig. 2From: Morphologically defined sub-stages of C. elegans vulval development in the fourth larval stageSub-stages of wild-type worms at specific time points after the L3-to-L4 lethargus. Sub-stages were determined every hour and also at 3.5, 5.5, 6.5, 8.5 and 9.5 h after synchronization. At least ten animals were observed every hour up to eight hours, and at least four animals were observed at all other time pointsBack to article page