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Fig. 5 | BMC Developmental Biology

Fig. 5

From: FOXL2 modulates cartilage, skeletal development and IGF1-dependent growth in mice

Fig. 5

FOXL2 expression in WT mouse at different stages of development. (a) RT-qPCR shows different levels of Foxl2 expression in tail, vertebral column and leg at 14.5 dpc and in skull vault at P0 and P7. (b) Schematic view of a 9.5 dpc mouse embryo. Red lines indicate the position of sections shown in c and d. (c-l) Localization by immunofluorescence confocal microscopy shows FOXL2 in green, and nuclei in red. (c,d) At 9.5 dpc FOXL2 expression is mainly observed in migrating mesenchymal cells (c) and in the caudal neural tube (d). (d1,d2) Magnification of the region boxed in d. (e) At 12.5 dpc FOXL2 expression is seen in the head near the nascent skull, where several FOXL2 positive cells are dispersed in the mesenchymal tissue. (f,g) Magnification of the region shown in e. Arrowhead in g indicates a migrating cell. (h) Pre-cartilagineous tissue of the first cervical vertebra. (i-l) At 14.5 dpc FOXL2 expression is revealed in vertebral column (i), ribs (j), hip (k), and finger (l). Nte, neural tube epithelium; hm, head mesenchyme; vb, vertebral bodies; im, intervertebral mesenchyme; r, ribs; h, hip; f, finger. Scale bars: (b) = 500 um, (c,d) 10X = 300 um, (d1,e,h,i-l) 40X = 75 um, (d2) 63X = 47,6 um, (f,g) 63X plus Digital Zoom 2.8X = 16.9 um

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