Table 1 List of antibodies and related dilution used
Name | Host | Provider | Dilution | Application |
|---|---|---|---|---|
anti-STRO-1 | mouse | Santa Cruz | 1: 50 | FACS, IF |
anti-c-Kit | rabbit | Santa Cruz | 1: 50 | FACS, IF |
anti-CD34 | mouse | Millipore | 1: 50 | FACS, IF |
anti-Runx2 | rabbit | Abcam | 1:100 | IF |
anti-osteopontin | mouse | Santa Cruz | 1:100 | IF |
anti-osterix | rabbit | GeneTex | 1:100 | IF |
anti-osteocalcin | mouse | Abcam | 1:100; 1:1000 | IF; WB |
anti-human collagen I | mouse | Millipore | 1:1000 | WB |
anti-human nuclei | mouse | Millipore | 1:100 | IF |
anti-human mitochondrial protein | mouse | Millipore | 1:100 | IF |
anti-myosin | rabbit | Sigma Aldrich | 1:100 | IF |
anti-β-III-Tubulin | rabbit | Cell Signaling | 1:100; 1:1000 | IF; WB |
anti-MAP2 | mouse | Sigma Aldrich | 1:100; 1:1000 | IF; WB |
anti-NeuN | mouse | Millipore | 1:100 | IF |
anti-Synapsin | rabbit | Cell Signaling | 1:100 | IF |
anti-GFAP | mouse | Cell Signaling | 1:100; 1:1000 | IF; WB |
anti-nestin | mouse | Millipore | 1:100 | IF |
anti-CD271 | mouse | BioLegend | 1:100 | IF |
anti-caspase 3 | rabbit | Sigma Aldrich | 1:1000 | WB |
anti-actin | goat | Santa Cruz | 1: 3000 | WB |