Figure 3

Reversible consequences of altered dietary composition on Notch signalling. (A) Time course of M7-lacZ expression in adults flies maintained post eclosion on St + Yeast. (B) Time course of Mβ-lacZ expression in adults flies maintained post eclosion on St + Yeast. (C) Time line of dietary shifts used in D, E. (D) M7-lacZ expression of adult flies maintained post eclosion on P/G-depleted medium or shifted to St + Yeast at 6 days post eclosion. (E) Time line of Mβ-lacZ expression of adult flies maintained post eclosion on P/G-depleted medium or shifted to St + Yeast at 6 days post eclosion. (F) M7-lacZ expression is reduced when flies are transferred to agar medium at eclosion for 3 days and restored if then shifted to St + Yeast for further 3 days. Data in A, B, D-F shown as % total ovarioles with strong, weak or no lacZ staining. Number of ovarioles examined indicated above each graph. *** p < 0.001 for indicated comparisons (Chi square test). (G,H) Adult flies were maintained for 15 days on St + Yeast before shifting to P/G-depleted medium or agar. Cap cells (G) and GSCs (H) decline in number after 6 days following nutrient downshift to both P/G-depleted and agar compared to St + Yeast controls. Data displayed as mean ± SEM * indicates p < 0.05; *** p < 0.001, Mann–Whitney U test, n = 21 to 60.