Figure 3

Transcriptome analysis clusters HR+ cells by developmental state. (A) Principal Component Analysis (PCA) was performed on three biological replicates of HR+ cells derived from virgin (Vir), 3-day (3D) and 7-day (7D) pregnant mice based on 19,821 probes (see Methods for details). A scatterplot of the first two principal components demonstrates a separation by pregnancy along the first principal component (PC1) and by duration of pregnancy along the second principal component (PC2). (B) Unsupervised hierarchical clustering based on 1328 probes (probes with an absolute fold-change > =2 and false discovery rate (FDR) of 10% or less) shows that the biological replicates cluster according to developmental state. (C) Venn diagram illustrating the number of genes that were changed more than 2-fold with a FDR of 10 in the 3 replicates of HR+ cells isolated at day 3 of pregnancy (3D, light grey) compared to virgin samples and the overlap of this gene set compared to differentially expressed genes at day 7 of pregnancy (7D, dark grey, more than 2 fold change compared to virgin).