Figure 5
Utilization of PIPES and HEPES result in defects in lumen morphology. (A–F) Immunofluorescence micrographs of 96 h w 1118 Drosophila ommatidium in cross-section view. The rhabdomeres, F-actin, are labeled with Phalloidin (magenta) and EYS is shown in green. The tissues are fixed in (A) PEM, (B) PEM + 10% PBST, (C) PIPES, (D) HEPES, (E) PBS, (F) PBST. Scale bar, 2 μm.